Diagnostic techniques for rapid detection of Vibrio species

Vibrio sp. has caused several foodborne outbreaks of gastroenteritis around the world. Given mounting evidence that poses a significant public health threat, and initiating effective treatment for infection requires rapid identification causative organism, there is need reliable, cost-effective point care diagnostic testing. Conventional detection techniques are current gold standard in detecting sp., typically resource-intensive, laborious, time-consuming. These limitations greatly hinder their efficiency under-developed countries, which usually regions most affected by infections. Thus, development rapid, reliable methods crucial to overcome conventional methods. This review aims summarize give insights into array focuses on different detect including polymerase chain reactions, loop-mediated isothermal amplification, enzyme-linked immunosorbent assays, microarrays, lateral flow immunoassays, aptasensors, spot dye-based sensors CRISPR/Cas-PCR systems. The were found have high specificity sensitivity various species within genus Vibrio. However, each method its own advantages limitations, thus continuous effort needed improve what currently available develop with sensitivity, specificity, affordability minimal obtain consistent accurate diagnoses.